NDRG2与GFAP在不同脑区星形胶质细胞的表达与分布

目的:观察NDRG2(N-myc下游调节基因2)与GFAP(胶质纤维酸性蛋白)在不同脑区星形胶质细胞的表达与分布。方法:利用免疫荧光NDRG2与GFAP双标技术以及Western Blot技术观察皮层、海马及纹状体等不同脑区星形胶质细胞NDRG2和GFAP的表达与分布。结果:免疫荧光结果显示NDRG2阳性细胞广泛而均匀地分布于不同脑区,并与GFAP存在较好的共定位;NDRG2与GFAP标记的星形胶质细胞形态不尽相同。Western Blot结果显示NDRG2在皮层中表达比海马和纹状体多,而GFAP在海马中表达比皮层和纹状体多。结论:NDRG2广泛表达于不同脑区星形胶质细胞,并于GFAP存在较好的共定位。

The Expression and Distribution of NDRG2 and GFAP in Astrocytes of Different Brain Regions

ABSTRACT Objective: To detect the expression and distribution of NDRG2(N-myc downstream regulated gene 2) and GFAP(glial fibrillary acidic protein) in astrocytes of different brain regions. Methods: Immunofluorescence staining and western blot were used to detect the expression and distribution of NDRG2 and GFAP in astrocytes of different brain regions, such as cortex, hippocampus, striatum and so on. Results: Double immunofluorescent staining results show that NDRG2 positive cells are widely and evenly distributed in different brain regions, and NDRG2 displays a strong colocalization with GFAP. The morphology of astrocytes labeled by NDRG2 and GFAP are not completely the same. Western Blot results show that the expression of NDRG2 protein in the cortex is higher than that in the hippocampus and striatum, while the expression of GFAP protein in hippocampus is the highest. Conclusion: NDRG2 is widely distributed in different brain regions and displays a strong colocalization with GFAP in astrocytes.