NDRG2在上皮性卵巢癌中的表达及与预后的关系

目的:探讨NDRG2在上皮性卵巢癌中的表达及其与预后的关系。方法:收集新鲜的上皮性卵巢癌和正常卵巢组织各15例,采用real-time PCR检测和比较其NDRG2 m RNA的表达。收集上皮性卵巢癌病理切片96例进行免疫组化检测其NDRG2蛋白的表达,并收集患者的临床病理资料,随访患者的生存情况,分析NDRG2蛋白的表达与上皮性卵巢癌患者临床病理特征和预后的关系。结果:上皮性卵巢癌组织中NDRG2 mRNA和蛋白的表达均显著低于正常卵巢组织(P0.05)。NDRG2在上皮性卵巢癌组织中的表达随着上皮性卵巢癌病理分期的升高而降低,而且其表达降低和患者不良预后显著相关(P=0.002),但其表达和不同上皮性卵巢癌的病理类型、分化程度以及年龄均无明显相关性。去除手术病理分期的影响,NDRG2表达下调和肿瘤细胞减灭术的满意程度以及术后规范化疗是三个影响上皮性卵巢癌预后的重要因素。结论:NDRG2在上皮性卵巢癌中的表达下降,并与患者的不良预后呈显著正相关。

Expression of NDRG2 in the Epithelial Ovarian Carcinoma and Its Influence on the Prognosis

ABSTRACT Objective: To study the expression of NDRG2 in epithelial ovarian cancer(EOC) and its correlation with the prognosis of EOC patients. Methods: 15 cases of fresh epithelial ovarian cancer and 15 cases of normal ovarian tissue were collected. The mRNA expression of NDRG2 were detected and compared by real-time PCR. 96 cases of epithelial ovarian cancer pathological sections were collected and the expression of NDRG2 protein was detected by immunohistochemistry, the clinicopathological characteristics and survival of patients were statistically analyzed. Results: The mRNA and protein expression of NDRG2 in the EOC tissue were significantly lower than those of the normal ovarian tissue(P<0.05). The protein expression of NDRG2 decreased with the increase of pathological staging of EOC and was significantly correlated with the poor prognosis of EOC patients(P=0.002). However, no obvious correlation was found of the protein expression of NDRG2 with pathological type, differentiated degree and age(P>0.05). Excluding the effect of surgical-pathological staging, decrease of NDRG2 expression, satisfaction of cytoreductive surgery and postoperative standard chemotherapy were influencing factors for the prognosis of EOC patients. Conclusion: The expression of NDRG2 was decreased in the EOC tissue and was positively correlated with theprognosis of EOC patients.