Programmed cell death at the periphery of the pupal wing of the butterfly,Pieris rapae |
| |
Authors: | R Kodama A Yohida T Mitsui |
| |
Institution: | (1) Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, 444 Myodaiji-cho, Okazaki, Japan;(2) Biohistory Research Hall, 1-1 Murasaki-cho, 569 Takatsuki, Japan;(3) Laboratory of Insect Toxicology and Physiology, Institute of Physical and Chemical Research (RIKEN), 351-01 Wako, Japan |
| |
Abstract: | The outline of the adult wing of lepidopteran insects (butterflies and moths) emerges as a result of disappearance of a group of cells at the periphery of the pupal wing. Histological observation of the pupal wing of Pieris rapae showed that, just after apolysis of the wing epithelium from the pupal cuticle, there occurs a rapid and localized decrease of the number of cells at the periphery of the wing. This decrease occurs through cell death, which lasts 1–1.5 days at 20°C. Dying cells lose contact with the neighbouring cells and show condensation of chromatin and cytoplasm. They then appear to be phagocytosed by neighbouring epithelial cells or discharged through the basal surface of the epithelium into the lumen within the wing and taken up by phagocytes. Fragmentation of DNA in the nuclei was detected in the dead cells or their debris. These results indicate that programmed cell death in the lepidopteran wing proceeds through a mechanism closely similar to that of apoptosis in the vertebrate. |
| |
Keywords: | Lepidopteran insect Butterfly wing Programmed cell death Apoptosis Wing morphogenesis |
本文献已被 SpringerLink 等数据库收录! |
|