A rapid purification procedure for pyruvate kinase from the hyphal fungus Aspergillus nidulans |
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| Authors: | H C Kester J H Uitzetter L H de Graaff J Visser |
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| Institution: | Department of Genetics, Agricultural University, Wageningen, The Netherlands. |
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| Abstract: | Pyruvate kinase was purified from the filamentous fungus Aspergillus nidulans with a 45-55% yield. The procedure involved dye-affinity chromatography and fast protein liquid chromatography, resulting in highly active and pure enzyme in milligram quantities within 2 days. The purified enzyme, a tetramer with a subunit molecular weight of 65,000 and an isoelectric point of 4.7, was used to determine the amino acid composition. |
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