| Abstract: | The involvement of kinases in heat stress signaling in tomato cells was studied by in gel kinase assays using myelin basic protein as substrate, and by in vitro phosphorylation assays in Mono Q fractions of tomato cell lysates. A kinase with an apparent molecular mass of approximately 50 kDa is rapidly deactivated upon heat stress as judged from in gel kinase assays. Cycloheximide treatment increases kinase activity, but concomitant heat treatment abolishes cycloheximide-induced activation. Kinase activity from untreated cells was recovered at about 130 and 250 mM NaCl from Mono Q columns. |
