Interactions of an azomercurial with proteins |
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| Authors: | HOROWITZ M G KLOTZ I M |
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| Affiliation: | 1. Case Western Reserve University School of Medicine, Cleveland, OH, USA;2. Medical University of South Carolina, Charleston, SC, USA;3. Rainbow Babies and Children''s Hospital, Cleveland, OH, USA;1. Department of Physics, University of Auckland, PB 92019, Auckland 1142, New Zealand;2. School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Queensland 4072, Australia |
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| Abstract: | With a colored mercurial, 4-(p-dimethylaminobenzeneazo)phenylmercuric acetate, it is possible to measure the thiol content of proteins from shifts in visible absorption spectra or from changes in optical density as mercurial is concentrated in an aqueous, protein-containing phase. The latter procedure is more precise and gives values of 0.66, 1.83, and about 4 for the number of moles of —SH per mole of bovine serum albumin, β-lactoglobulin, and ovalbumin, respectively. Differences in reactivity of thiols can also be distinguished. |
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