Use of glnQ as a counterselectable marker for creation of allelic exchange mutations in group B streptococci |
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| Authors: | Tamura Glen S Bratt Debra S Yim Harry H Nittayajarn Aphakorn |
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| Affiliation: | Division of Infectious Disease and Department of Pediatrics, Children's Hospital and Regional Medical Center and University of Washington, 4800 Sand Point Way N.E., Seattle, WA 98105, USA. gtamura@u.washington.edu |
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| Abstract: | Efficient allelic exchange mutagenesis in group B streptococci (GBS) has been hampered by the lack of a counterselectable marker system. Growth inhibition of GBS by the glutamine analog gamma-glutamyl hydrazide requires glnQ. We have used this phenomenon to create a counterselectable marker system for efficient selection of allelic exchange mutants in GBS. |
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