Finite life span of hybrids formed by fusion of different simian virus 40-immortalized human cell lines. |
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| Authors: | N J Whitaker E L Kidston R R Reddel |
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| Affiliation: | Children's Medical Research Foundation, Camperdown, New South Wales, Australia. |
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| Abstract: | Simian virus 40 (SV40) genes are able to induce immortalization of normal human cells after a culture crisis during which unknown cellular genetic changes presumably occur. To determine whether these genetic changes are always identical, we performed somatic cell hybridization analysis of an SV40-immortalized human bronchial epithelial cell line, BET-1A. Fusion of BET-1A with an SV40-immortalized fibroblast cell line resulted in hybrids that senesced, indicating that these cell lines are in different complementation groups for immortalization. |
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