Augmentation of DNA vaccine potency through secretory heat shock protein-mediated antigen targeting |
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Authors: | Hauser Hanns Chen Si-Yi |
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Institution: | Institute of Immunology, University of Vienna, and Children's Cancer Research Institute, St. Anna Kinderspital, Vienna, Austria. |
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Abstract: | In an effort to enhance the potency of DNA vaccines, we have developed a new strategy to increase antigen presentation by dendritic cells, one that results in markedly improved cytotoxic T-lymphocyte responses, antibody production, and antitumor effects in vivo. Here, we present the rationale and design of a vaccine encoding a secreted antigen-heat shock protein 70 fusion molecule, targeted to the MHC class I cross-presentation pathway of dendritic cells. Using the human papilloma virus 16 E7 protein as a model antigen, we illustrate the preparation of this vaccine and the main experimental procedures used to test such constructs. |
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