Plantlet regeneration of <Emphasis Type="Italic">Kappaphycus alvarezii</Emphasis> var. adik-adik by tissue culture |
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Authors: | A Q Hurtado A B Biter |
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Institution: | (1) Aquaculture Department Southeast Asian Fisheries Development Center, Tigbauan, Iloilo, 5021, Philippines;(2) Integrated Services for the Development of Aquaculture and Fisheries (ISDA), McArthur Highway Tabuc Suba, Jaro Iloilo, 5000, Philippines;(3) Baylor College of Medicine, Dallas, TX, USA |
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Abstract: | Three color morphotypes of Kappaphycus alvarezii var. adik-adik (brown, green and red) collected from a farming area in Tictauan Is., Zamboanga City, Philippines were used as explants
in the study in order to micropropagate ‘new’ plants. Individual sections of sterile Kappaphycus alvarezii var. adik-adik, initially cultured in a 48-well culture plate containing ESS/2 + E3 + PGR, released callus cells after 4–5 days of incubation
at 23–25°C, 13:11H LD cycle and 10–15 μmol photons m−2 s−1 light intensity. True calli were formed after 29–35 days following dense formation of filaments or undifferentiated round
cells at the medullary and inner cortical layers of the section. Plantlets (2–3 mm long) of Kappaphycus alvarezii var. adik-adik were able to regenerate after 98, 150 and 177 days in-vitro among the reds, greens, and browns, respectively. This study established successful methods for the production and regeneration
of tissue explants of Kappaphycus alvarezii var. adik-adik which can possibly be used to mass produce ‘new’ cultivars for land- and sea-based nurseries as sources for commercial farming.
Presented at the 6th Meeting of the Asian Pacific Society of Applied Phycology, Manila, Philippines. |
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Keywords: | Callus Kappaphycus Plantlet regeneration Tissue culture |
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