Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway |
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Authors: | Min Huang Fred Bernd Oppermann Alexander Steinbüchel |
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Institution: | Department of Biology, Sichuan Normal University, Chengdu, China; Institut für Mikrobiologie der Georg-August-Universität zu Göttingen, Göttingen, Germany |
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Abstract: | Abstract The 2,3-butanediol dehydrogenase and the acetoin-cleaving system were simultaneously induced in Pseudomonas putida PpG2 during growth on 2,3-butanediol and on acetoin. Hybridization with a DNA probe covering the genes for the E1 subunits of the Alcaligenes eutrophus acetoin cleaving system and nucleotide sequence analysis identified acoA (975 bp), acoB (1020 bp), acoC (1110 bp), acoX (1053 bp) and adh (1086 bp) in a 6.3-kb genomic region. The amino acid sequences deduced from acoA , acoB , and acoC for E1α ( M r 34639), E1β ( M r 37268), and E2 ( M r 39613) of the P. putida acetoin cleaving system exhibited striking similarities to those of the corresponding components of the A. eutrophus acetoin cleaving system and of the acetoin dehydrogenase enzyme system of Pelobacter carbinolicus and other bacteria. Strong sequence similarities of the adh translational product (2,3-butanediol dehydrogenase, M r 38361) were obtained to various alcohol dehydrogenases belonging to the zinc- and NAD(P)-dependent long-chain (group I) alcohol dehydrogenases. Expression of the P. putida ADH in Escherichia coli was demonstrated. The aco genes and adh constitute presumably one single operon which encodes all enzymes required for the conversion of 2,3-butanediol to central metabolites. |
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Keywords: | 2 3-Butanediol dehydrogenase Acetoin-cleaving system Acetoin dehydrogenase enzyme system Pseudomonas putida Alcaligenes eutrophus Pelobacter carbinolicus |
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