Determination of ergot alkaloids in rye and rye flour |
| |
Authors: | C Müller H S Klaffke W Krauthause R Wittkowski |
| |
Institution: | (1) Federal Institute for Risk Assessment, Thielallee 88–92, 14195 Berlin, Germany;(2) Federal Institute for Risk Assessment, Thielallee 88-92, 14195 Berlin, Germany |
| |
Abstract: | An effective and timesaving analytical method was developed for the determination of 12 ergot alkaloids (ergometrine, ergotamine,
ergocristine, α-ergokryptine, ergosine, ergocornine, and their respective -inine isomers) in rye and rye flour. Samples were
extracted with dichloromethane/ethyl acetate/methanol/aqueous ammonia (25%) (50/25/5/1, v/v/v/v), and extracts were purified
using a basic alumina column. The eluate was dried in the nitrogen stream and redissolved in acetonitrile/ ammonia carbamate-buffer
(0.2 g/1), (1/1, v/v), and injected into an HPLC-FLD system (λEx 330 nm, λEm 415 nm), using the same mixture as mobile phase and a Phenyl-Hexyl column. Detection limits for the individual compounds
ranged from 0.01 μg/kg to 0.5 μg/kg. In sample material spiked with a mixture of these compounds at two different levels (13
μg/kg and 27 μg/kg per compound), mean (n=5) recoveries were at 101% (sr 6.4%) and 89% (sr 3.1%), respectively.
Presented at the 28th Mykotoxin-Workshop, Bydgoszcz, Poland, May 29–31, 2006 |
| |
Keywords: | Claviceps purpurea ergot alkaloids rye HPLC-FLD |
本文献已被 SpringerLink 等数据库收录! |
|