Transgenic markers for mammalian chimeras |
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Authors: | James Alexander Thomson Davor Solter |
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Affiliation: | (1) The Wistar Institute, 36th at Spruce Street, 19104 Philadelphia, PA, USA |
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Abstract: | Summary We describe the construction of aggregation chimeras between normal and transgenic embryos containing multiple copies of mouse -globin genes. The transgenic component of the chimeras is then detected in tissue sections by a DNA-DNA in situ hybridization technique, using a biotinylated DNA -globin probe and an avidin-linked alkaline phosphatase detection system. The general advantages of transgenic markers for chimeras are discussed. |
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Keywords: | Chimeras In situ hybridization Mice Transgenic |
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