A nuclear protein associated with cell divisions in plants |
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Authors: | J. A. Smith M. R. Krauss C. Borkird Z. Renee Sung |
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Affiliation: | (1) Department of Genetics, University of California, 94720 Berkeley, CA, USA;(2) Department of Plant Pathology, University of California, 94720 Berkeley, CA, USA;(3) Present address: Eni-Chem Americas Biotechnology, Corporate Center, 2000,Princeton Park, 08852 Monmouth Junction, NJ, USA;(4) Present address: Protein Database, Inc., 405 Oakwood Rd., 11746 Huntington Station, NY, USA;(5) Present address: Laboratorium voor Genetica, Rijksuniversiteit, B-9000 Gent, Belgium |
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Abstract: | A nuclear protein, present in carrot meristems and rapidly proliferating cultured cells of carrot (Daucus carota L.) has been identified by the use of a monoclonal antibody (MAb 21D7). By combining the techniques of two-dimensional polyacrylamide gel analysis and blotting separated proteins onto nitrocellulose sheets, it was shown that the antibody detected a single polypeptide of apparent molecular mass (Mr) of 45000 and an isoelectric focusing point (pI) of 6.7. This protein was found by subcellular fractionation and immunofluorescence to be highly concentrated in the nucleoli of somatic and zygotic embryos of a wide range of plants. It was not detectable in logarthmically growing cells ofEscherichia coli, yeast, embryos ofDrosophila melanogaster or cultured C3H mouse cells. These data indicate that this protein is a highly conserved non-histone protein associated with nuclei of rapidly dividing plant cells.Abbreviations Mr apparent molecular mass - Da dalton - Ig immunoglobulins - MAb monoclonal antibody - SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis - 2-D gel two-dimensional gel electrophoresis - 2,4-D 2,4-dichlorophenoxyacetic acid |
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Keywords: | Daucus (somatic embryo) Cell division (protein) Embryo (somatic zygotic) Immunofluorescence, indirect Meristem (cell divisions) Monoclonal antibody Nuclear protein (non-histone) Protein (nuclear) |
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