Oxidation of phenols by horseradish peroxidase and lactoperoxidase compound II--kinetic considerations. |
| |
| Authors: | M S Zahida W Deva G M Peerzada D V Behere |
| |
| Affiliation: | Department of Chemical Sciences, Tata Institute of Fundamental Research, Navy Nagar, Bombay. |
| |
| Abstract: | Oxidation of para substituted phenols by horseradish peroxidase compound II (HRP-II) and lactoperoxidase compound II (LPO-II) were studied using stopped flow technique. Apparent second order rate constants (kapp) of the reactions were determined. The kinetics of oxidation of phenols by HRP-II and LPO-II have been compared with the oxidation potentials of the substrates. Reorganization energies of electron-transfer of phenols to the enzymes were estimated from the variation of second order rate constants with the thermodynamic driving force. |
| |
| Keywords: | |
|
