Response of light- and dark-grown callus of atrazine-resistant and susceptible rapeseed (Brassica napus) to varying concentrations of atrizine |
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Authors: | Ian Grant Wallace D Beversdorf Julia Zilka |
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Affiliation: | 1. Department of Crop Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada
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Abstract: | Callus cultures initiated with tissue of triazine-resistant and susceptible lines of Brassica napus L. were cultured in the presence of atrazine (10-5 to 10-4 M) under a 16-h photoperiod or in continuous darkness. On atrazine-free media callus growth was characteristically greater for the light-grown cultures. On media containing atrazine, growth of callus from triazine-resistant and susceptible rapeseed cultured in the dark did not differ significantly. Addition of atrazine to the culture medium resulted in a significant reduction in the growth of light-grown callus of the susceptible genotype to a level equivalent to that of the dark-grown cultures. Atrazine had no effect on the growth of light-grown callus initiated with triazine-resistant rapeseed. |
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