Rapid multiplication of Sapium sebiferum Roxb. by tissue culture |
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Authors: | M Kotwal Mridula P K Gupta A F Mascarenhas |
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Institution: | 1. Biochemistry Division, National Chemical Laboratory, 411 008, Poona, India
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Abstract: | Multiple shoots formation and elongation was induced from stem explants of Sapium seedlings on media containing cytokinins. Leaf explants produced callus on a medium containing cytokinins, auxin, casein hydrolysate and coconut milk, which could be induced to form multiple shoots on transfer to a medium lacking casein hydrolysate, coconut milk and auxin. Rooting of isolated shoots by treatment with an auxin mixture (indole-3-acetic acid, indole-3-butyric acid and indole-3-propionic acid) and transfer of the plantlets to field have also been successful. |
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