Development of ten highly polymorphic microsatellite markers for Sunbirds (Aves: Nectariniidae) with broad cross-amplification utility in at least eight species |
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| Authors: | Rauri C K Bowie Anna B Sellas Kevin A Feldheim |
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| Institution: | (1) Museum of Vertebrate Zoology and Department of Integrative Biology, University of California, 3101 Valley Life Science Building, Berkeley, CA 94720, USA;(2) DST/NRF Centre of Excellence at the Percy FitzPatrick Institute, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa;(3) Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605, USA |
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| Abstract: | We describe the isolation of ten microsatellite loci from the Rwenzori Double-collared Sunbird using an enrichment protocol.
All loci were variable with the number of alleles ranging from 5 to 13, and observed heterozygosity ranging from 0.500 to
0.929. Nine of these loci were screened on a panel of seven additional Sunbird species from three genera. All nine were successfully
amplified and remained highly variable. Given 10–15% sequence divergence among these species in the mtDNA ND2 gene, our results
suggest that these markers will work broadly across most members of the species rich bird family Nectariniidae. |
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