HPLC-ELSD法分析木薯韧皮部汁液主要糖成分

采用高效液相色谱—蒸发光散射检测法(HPLC-ELSD)对木薯韧皮部汁液糖成分进行了分析.结果表明:与大多数木本植物一样,其同化物的主要运输形式是蔗糖,并未发现糖醇类和棉子糖等寡糖.对块根产量、淀粉含量较低的半野生种W14的对比实验发现,无论是蔗糖还是己糖含量都大大地低于栽培种,说明蔗糖是木薯块根淀粉累积的主要来源,对其累积速率和量起着决定性作用.结果同时证明此方法可以高效、快速简便地定性和定量测定木薯韧皮部汁液中的糖类.

Analysis of sugars in cassava phloem sap by HPLC-ELSD detection

High Performance Liquid Chromatography(HPLC) coupled with Evaporative Light Scattering Detection(ELSD) method was used to analyse the sugar contents in cassava phloem sap.The results showed that the main sugar was sucrose which was similar to other woody plants,no other sugar alcohols and raffinose family of oligosaccharides were found.W14 was an ancestral semi-wild species of cassava with low productivity and starch content,which was used as a control in this research.It was found that both of the sucrose and hexoses of W14 were less than the cultivar largely,which indicated that sucrose was the main source of starch accumulation of cassava tuber root,and it determined the rate and quantity of starch accumulation.Meanwhile,and it proved that this method could determine the nature and quantity of sugars in cassava phloem sap efficiently,rapidly and conveniently.