MiR‐376a suppresses the proliferation and invasion of non‐small‐cell lung cancer by targeting c‐Myc |
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| Authors: | Youyu Wang Wei Cong Gang Wu Xueming Ju Zhixi Li Xin Duan Xueli Wang Hong Gao |
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| Institution: | 1. Department of Thoracic Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu, Sichuan, China;2. Department of Hepatobiliary Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu, Sichuan, China;3. Department of Ultrasound, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu, Sichuan, China;4. Department of Pediatric Surgery, Hospital of The University of Electronic Science and Technology of China and Sichuan Provincial People's Hospital, Chengdu, Sichuan, China;5. Department of Orthopaedics, Chengdu First People's Hospital, Chengdu, Sichuan, China;6. Department of Respiration Medicine, Pidu District People's Hospital, Chengdu, Sichuan, China |
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| Abstract: | It has been reported that miR‐376a is involved in the formation and progression of several types of cancer. However, the expression and function of miR‐376a is still unknown in non‐small cell lung carcinomas (NSCLC). In this study, the expression of miR‐376a in NSCLC tissues and cell lines were examined by real‐time PCR, the effects of miR‐376a on cell proliferation, apoptosis and invasion were evaluated in vitro. Luciferase reporter assay was performed to identify the targets of miR‐376a. The results showed that miR‐376a was significantly downregulated in NSCLC tissues and cell lines. Restoration of miR‐376a in NSCLC cell line A549 significantly inhibited cell proliferation, increased cell apoptosis and suppressed cell invasion, compared with control‐transfected A549 cells. Luciferase reporter assay showed that c‐Myc, an oncogene that regulating cell survival, angiogenesis and metastasis, was a direct target of miR‐376a. Over‐expression of miR‐376a decreased the mRNA and protein levels of c‐Myc in A549 cells. In addition, upregulation of c‐Myc inhibited miR‐376a‐induced inhibition of cell proliferation and invasion in A549 cells. Therefore, our results indicate a tumor suppressor role of miR‐376a in NSCLC by targeting c‐Myc. miR‐376a may be a promising therapeutic target for NSCLC. |
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| Keywords: | c‐Myc miR‐376a NSCLC tumor suppressor |
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