UHPLC‐HRMS/MS Based Profiling of Algerian Lichens and Their Antimicrobial Activities |
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Authors: | Rafika Brakni Monia Ali Ahmed Pauline Burger Aurélie Schwing Grégory Michel Christelle Pomares Lillia Hasseine Laurent Boyer Xavier Fernandez Anne Landreau Thomas Michel |
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Institution: | 1. Département de Biologie, Laboratoire de Biologie Végétale et Environnement, Université Badji‐Mokhtar, Annaba, Algeria;2. Université C?te d'Azur, CNRS, Institut de Chimie de Nice, UMR 7272, Nice Cedex 2, France;3. Université C?te d'Azur, C3M Inserm, Nice Cedex 3, France;4. Service de Parasitologie‐Mycologie, Centre Hospitalier Universitaire de Nice, Nice Cedex 3, France;5. Université d'Angers, Université Bretagne ‐ Loire, Faculté de santé, Département pharmacie, 16 bd Daviers, Angers cedex 01, France |
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Abstract: | Lichens are complex symbiotic organisms able to produce a vast array of compounds. The Algerian lichen diversity has only prompted little interest even given the 1085 species listed. Herein, the chemodiversity of four Algerian lichens including Cladonia rangiformis, Ramalina farinaceae, R. fastigiata, and Roccella phycopsis was investigated. A dereplication strategy, using ultra high performance liquid chromatography‐high resolution‐electrospray ionization‐mass spectrometry (UHPLC‐HRMS/MS), was carried out for a comprehensive characterization of their substances including phenolics, depsides, depsidones, depsones, dibenzofurans, and aliphatic acids. Some known compounds were identified for the first time in some species. Additionally, the lichenic extracts were evaluated for their antifungal and antimicrobial activities on human pathogenic strains (Candida albicans, C. glabrata, Aspergillus fumigatus, Staphylococcus aureus, and Escherichia coli). Cyclohexane extracts were found particularly active against human pathogenic fungi with MIC80 values ranging from 8 to 62.5 μg/mL, without cytotoxicity. This study highlights the therapeutic and prophylactic potential of lichenic extracts as antibacterial and antifungal agents. |
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Keywords: | algerian lichens UHPLC‐HRMS dereplication antifungal activity antibacterial activity |
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