Toxicological Aspects of the Essential Oil from Cinnamodendron dinisii |
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| Authors: | Milene A. Andrade Maria das Graças Cardoso Paulo S. C. Preté Maurílio J. Soares Camila M. O. de Azeredo Marcus V. C. Trento Mariana A. Braga Silvana Marcussi |
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| Affiliation: | 1. Organic Chemistry Laboratory, Department of Chemistry, Federal University of Lavras, Lavras, ‐ MG, Brazil;2. Cell Biology Department, Universidade de Brasília, Brasília, – DF, Brazil;3. Biochemistry Laboratory, Department of Chemistry, Federal University of Lavras, Lavras, ‐ MG, Brazil;4. Instituto Carlos Chagas (ICC)/Fiocruz, Curitiba, ‐ PR, Brazil |
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| Abstract: | The objective of this study was to determine cytotoxic activity, hemolytic activity, and to evaluate the ability of the essential oil from Cinnamodendron dinisii to induce DNA fragmentation of human lymphocytes. The essential oil was obtained by hydrodistillation. Cytotoxic activity was determined by the MTT method. Hemolytic activity was evaluated by spectrophotometric quantification of hemoglobin released by erythrocytes. Damage to lymphocyte DNA molecules was assessed by the Comet assay. The essential oil under study showed high cytotoxic activity on Vero cells (CC50 = 35.72 μg/mL) and induced hemolysis in both hematocrits, besides leading to the oxidation of hemoglobin released. The genotoxic activity of C. dinisii essential oil was also observed, which induced concentration‐dependent DNA fragmentation of human lymphocytes and, at 50 μL/mL, it was more active than the positive control. The essential oil from C. dinisii has a toxic action, suggesting a special attention in the application of this oil to health‐promoting activities; however, among its components, there are molecules with potential for future application in anticancer therapies. |
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| Keywords: | cytotoxicity genotoxicity hemolysis human lymphocytes volatile oils |
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