Biobutanol production from rice bran and de-oiled rice bran by <Emphasis Type="Italic">Clostridium saccharoperbutylacetonicum</Emphasis> N1-4

Rice bran (RB) and de-oiled rice bran (DRB) have been treated and used as the carbon source in acetone–butanol–ethanol (ABE) production using Clostridium saccharoperbutylacetonicum N1-4. The results showed that pretreated DRB produced more ABE than pretreated RB. Dilute sulfuric acid was the most suitable treatment method among the various pretreatment methods that were applied. The highest ABE obtained was 12.13 g/L, including 7.72 g/L of biobutanol, from sulfuric acid. The enzymatic hydrolysate of DRB (ESADRB), when treated with XAD-4 resin, resulted in an ABE productivity and yield of 0.1 g/L h and 0.44 g/g, respectively. The results also showed that the choice of pretreatment method for RB and DRB is an important factor in butanol production.