Apoptosis,necrosis and autophagy are influenced by metabolic energy sources in cultured rat spermatocytes |
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Authors: | Ximena Bustamante-Marín Clara Quiroga Sergio Lavandero Juan G Reyes Ricardo D Moreno |
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Institution: | 1.Departamento de Fisiología, Facultad de Ciencias Biológicas,Pontificia Universidad Católica de Chile,Santiago,Chile;2.Centro Estudios Moleculares de la Celula, Facultad Ciencias Quimicas y Farmaceuticas/Facultad Medicina,Universidad de Chile,Santiago,Chile;3.Department of Internal Medicine (Cardiology Division),University of Texas Southwestern Medical Center,Dallas,USA;4.Instituto de Química, Pontificia Universidad Católica de Valparaíso,Valparaíso,Chile;5.Departamento Biomédico, Facultad de Ciencias de la Salud,Universidad de Antofagasta,Antofagasta,Chile |
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Abstract: | Apoptosis, necrosis and autophagy are mechanistically related processes that control tissue homeostasis and cell survival.
In the testis, germ cell death is important for controlling sperm output, but it is unknown whether or not germ cells can
switch from apoptosis to necrosis, as has been reported in other tissues. Furthermore, autophagy has not been reported in
spermatogenesis. Spermatocytes (meiotic cells) and spermatids (haploid cells) use lactate rather than glucose as their primary
substrate for producing ATP. The metabolism of glucose, but not lactate, reduces ATP levels and increases intracellular H+] and Ca2+], both of which are associated with apoptosis and/or necrosis in somatic cells. In this work, we evaluated whether different
energy sources, such as lactate or glucose, can influence spermatocyte death type and/or survival in primary cultures. Spermatocytes
cultured for 12 h without an energy source died by necrosis, while spermatocytes cultured with 5 mM glucose showed a significant
increase in apoptosis, as evidenced by caspase activity, TUNEL assay and phosphatidylserine exposure. Apoptosis was not observed
in spermatocytes cultured with 5 mM lactate or deoxyglucose. Authophagy markers, such as LC3-II and autophagosomes, were detected
after 12 h of culture, regardless the culture conditions. These results suggest that the availability of glucose and/or lactate
affect the type of death or the survival of primary spermatocytes, where glucose can induce apoptosis, while lactate is a
protective factor. |
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