Phenomenon of selective staining of pericentromeric heterochromatin regions in chromosomes from spontaneously dividing cells by the acridine orange fluorochrome |
| |
Authors: | T V Kuznetzova I L Trofimova M S Lyapunova E V Evdokimenko V S Baranov |
| |
Institution: | 1.Ott Institute of Obstetrics and Gynecology, Northwestern Branch,Russian Academy of Medical Sciences,St. Petersburg,Russia;2.Department of Genetics and Breeding,St. Petersburg State University,St. Petersburg,Russia |
| |
Abstract: | A novel phenomenon of unusual selective acridine orange (AO) staining of pericentromeric heterochromatin regions (HRs) in
chromosomal preparations from tissue with known spontaneous mitotic activity (chorionic villi, placenta, embryonic tissues,
bone marrow, and testes), as well as embryonic stem cells, is described. Staining with 0.01% AO in a citric-phosphate (pH
5.5) or sodium phosphate (pH 7.0) buffer solution allows the HRs of human chromosomes (1q12, 9q12, 13p11.2, 14p11.2, 15p11.2,
16q11.2, 21p11.2, 22p11.2, and Yq12) and pericentromeric HRs of mouse chromosomes to be reliably detected by the red fluorescence
of AO. This method of AO staining does not require any pretreatment. Explanations for metachromatic AO staining of polymorphic
pericentromeric HRs in chromosomes of spontaneously dividing cells are suggested. A high reproducibility of the specific AO
staining makes it possible to suggest its using as a reliable quick method for detection of polymorphic HRs of human chromosomes
in cytogenetic prenatal diagnosis and oncohematology. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|