Phytosulfokine stimulates cell divisions in sugar beet (Beta vulgaris L.) mesophyll protoplast cultures |
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| Authors: | Ewa Grzebelus Marek Szklarczyk Joanna Greń Katarzyna ?niegowska Magdalena Jopek Iwona Kacińska Katarzyna Mro?ek |
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| Institution: | (1) Department of Genetics, Plant Breeding and Seed Science, Faculty of Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, 31-425 Krakow, Poland; |
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| Abstract: | The aim of this work was to improve plating efficiency of sugar beet mesophyll protoplast cultures. Preliminary experiments
showed that cultures of good quality, viable protoplasts were obtained in rich media based on the Kao and Michayluk formulation
and with the calcium alginate as an embedding matrix. Nevertheless, in these cultures cell divisions were either not observed
or very seldom confirming earlier reported recalcitrance of sugar beet protoplasts. The recalcitrant status of these cultures
was reversed upon application of exogenous phytosulfokine (PSK)—a peptidyl plant growth factor. The highest effectiveness
of PSK was observed at 100 nM concentration. Plating efficiencies obtained in the presence of PSK reached approximately 20%
of the total cultured cells. The stimulatory effect of phytosulfokine was observed for all tested breeding stocks of sugar
beet. Our data indicate that PSK is a powerful agent able to overcome recalcitrance of plant protoplast cultures. |
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