Stability of a Fusarium solani pisi recombinant cutinase in phosphatidylcholine reversed micelles |
| |
Authors: | A. M. Pinto-Sousa J. M. S. Cabral M. R. Aires-Barros |
| |
Affiliation: | (1) Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Centro de Engenharia Biológica e Química, 1000 Lisboa, Portugal |
| |
Abstract: | Summary A Fusarium solani pisi recombinant cutinase solubilized in phosphatidylcholine/isooctane reversed micelles was used to catalyse the esterification reaction of butyric acid with 2-butanol at pH 10.7. The influence of temperature, Wo and substrates on lipase stability was evaluated. The enzyme displays a better stability, with a half-life over 125 days, at a temperature of 22°C and for a low water content (WO= 6.5). Butyric acid increased the cutinase deactivation (t1/2=0.56h), while 2-butanol led to a similar half-life (t1/2=14h) as without substrate. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|