Isolation and purification of bovine testicular hyaluronidase |
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| Authors: | Barsukov A K Kozhevnikova O V Khokhriakova A V |
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| Affiliation: | Udmurt State University, Izhevsk, 426034 Russia. |
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| Abstract: | We developed a single-step method for chromatographic purification of the hyaluronidase extract on Sepharose blue, which produced high yield of the enzyme (95%) with high purity (40). Purification of hyaluronidase allowed us to obtain biological preparations standardized by the protein composition and enzyme activity and useful for basic and applied researches. |
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