A novel method of identifying genetic mutations using an electrochemical DNA array |
| |
Authors: | Wakai Junko Takagi Atsuko Nakayama Masato Miya Takahito Miyahara Takatoshi Iwanaga Tsuyoshi Takenaka Shigeori Ikeda Yasuyuki Amano Masahiko Urata Tomohiro |
| |
Institution: | TUM Gene, Inc., 3-1 Kazusa-Koito Kimitsu, Chiba 292-1149 Japan. |
| |
Abstract: | We describe the development of a new type of DNA array chip that utilizes electrochemical reactions and a novel method of simultaneously identifying multiple genetic mutations on an array chip. The electrochemical array (ECA) uses a threading intercalator specific to double-stranded nucleotides, ferrocenylnaphthalene diimide (FND), as the indicator. ECA does not require target labeling, and the equipment is simple, durable and less expensive. The simultaneous multiple mutation detection (SMMD) system using an ECA chip and FND utilizes an enzyme to simultaneously distinguish several genetic mutations such as single nucleotide polymorphism (SNP), insertion, deletion, translocation and short tandem repeat. We examined this SMMD system using an ECA chip, by detecting seven different mutations on the lipoprotein lipase (LPL) gene for 50 patients in a blind test. It turned out that all the results obtained were concordant with the sequencing results, demonstrating that this system is a powerful tool for clinical applications. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|