Changes of Curdlan Biosynthesis and Nitrogenous Compounds Utilization Characterized in <Emphasis Type="Italic">ntrC</Emphasis> Mutant of <Emphasis Type="Italic">Agrobacterium</Emphasis> sp. ATCC 31749 |
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Authors: | Li-Jun Yu Jian-Rong Wu Zhi-Yong Zheng Xiao-Bei Zhan Chi Chung Lin |
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Institution: | (1) Key Lab of Carbohydrate Chemistry & Biotechnology, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China; |
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Abstract: | The regulatory function of global regulator NtrC on curdlan biosynthesis and nitrogen consumption under nitrogen-limited condition
in Agrobacterium sp. ATCC 31749 was investigated. The ntrC mutant of Agrobacterium sp. was constructed by homologous recombination. The ability to utilize NH4Cl and KNO3 was impaired in the mutant. Other nitrogenous compounds, such as glutamic acid and glutamine, were utilized normally. Curdlan
production capability was impaired severely in the mutant. Curdlan production was 5-fold lower than the wild type strain in
batch fermentation with NH4Cl as the sole nitrogen source. However, up to 6.5 g l−1 of a newly found alkali-insoluble biopolymer was produced by the ntrC mutant when glutamic acid was used as nitrogen source. The new biopolymer had glycosidic bond and hydroxyl group but no β-configuration
absorption peak on IR spectrum was found as different from curdlan. In addition, the mutant exhibited a rapid morphological
change from the dot to rod form. These results deduced that the global regulator NtrC was involved in curdlan and other biopolymer
biosynthesis in Agrobacterium sp. ATCC 31749 in response to nitrogen-limited condition. |
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