首页 | 本学科首页   官方微博 | 高级检索  
     

草菇GH61家族基因的生物信息学分析及金属离子对其表达水平的影响
引用本文:饶佳,鲍大鹏,李燕,汪滢,程向阳,王莹. 草菇GH61家族基因的生物信息学分析及金属离子对其表达水平的影响[J]. 菌物学报, 2016, 35(5): 586-596. DOI: 10.13346/j.mycosystema.150014
作者姓名:饶佳  鲍大鹏  李燕  汪滢  程向阳  王莹
作者单位:1国家食用菌工程技术研究中心 农业部南方食用菌资源利用专业重点实验室 上海市农业遗传育种重点开放实验室 上海市农业科学院食用菌研究所 上海 201403; 2上海海洋大学食品科学与工程学院 上海 201306
基金项目:上海市科技人才计划项目(13XD1424700) Supported by Shanghai Science and Technology Talent Project (13XD1424700).
摘    要:在前期工作中发现草菇含有30个GH61家族基因同源物(Vv_gh61_1至Vv_gh61_30),进一步分析了这些基因的结构特点以及其编码蛋白基本性质和系统进化关系,并研究了Cu2+和Mn2+对基因表达水平的影响。分析表明有17个草菇GH61基因串联成6个基因簇,存在明显的串联重复现象,系统发育树与基因外显子位置分析表明串联重复基因分布在同一进化分枝上并具有相似的基因结构,串联重复基因编码序列一致性在71%–94%之间。草菇GH61编码蛋白的氨基酸数目在217–442aa之间,分子量和等电点分别介于22.4–45.4kDa和5.2–9.3之间,绝大多数都含有信号肽并定位在细胞外,都含有Glyco_hydro_61功能域以及CBM1、peroxidase等多样化的功能域,系统进化树表明草菇GH61家族基因具有3个主要进化分枝,与灰盖鬼伞等的GH61基因有较近的进化关系。金属离子诱导作用显示,Mn2+对草菇GH61家族基因的表达水平存在诱导作用而Cu2+的诱导作用不明显。

关 键 词:草菇  GH61家族  串联重复  基因表达  

Bioinformatic and gene expression analyses of the GH61 family genes of Volvariella volvacea
RAO Jia,BAO Da-Peng,LI Yan,WANG Ying,CHENG Xiang-Yang,WANG Ying. Bioinformatic and gene expression analyses of the GH61 family genes of Volvariella volvacea[J]. Mycosystema, 2016, 35(5): 586-596. DOI: 10.13346/j.mycosystema.150014
Authors:RAO Jia  BAO Da-Peng  LI Yan  WANG Ying  CHENG Xiang-Yang  WANG Ying
Affiliation:1National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; 2College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China
Abstract:Thirty putative GH61 family genes (Vv_gh61_1 to Vv_gh61_30) in the genome of Volvariella volvacea were identified previously. In this study, the genetic structure of these genes as well as their encoded proteins and phylogenetic relationships were reanalyzed. The effect of bivalent copper and manganese ions on the gene expression level was also investigated. The results showed that 17 out of 30 GH61 family genes distributed in 6 gene clusters with the form of tandem duplication. Analysis of phylogenetic trees and exon position reveals that these genes of the same cluster share the final phylogenetic clades and have similar gene structure, and most of amino acid sequences encoded by the genes in the same cluster have high identities (71%–94%). GH61 proteins of V. volvacea have 217–422aa in length, and their molecular weight and isoelectric point range at 22.4–45.4kDa and 5.2–9.3, respectively. The majority of GH61 proteins of V. volvacea have the signal peptides and thereby are secretable. The functional domains of Glyco_hydro_61, CBM1 and peroxidase are found in GH61 proteins of V. volvacea. Phylogenetic tree shows that GH61 family genes of V. volvacea cluster in three major clades with closely evolutionary relationships to those of Coprinopsis cinerea. The expressional levels of GH61 family genes increase while the genes are induced by Mn2+ at 100μmol/L, but induction effect of Cu2+ is indistinctive.
Keywords:Volvariella volvacea  GH61 family  tandem duplication  gene expression
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《菌物学报》浏览原始摘要信息
点击此处可从《菌物学报》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号