| 黄柄钙皮菌无菌培养条件的优化筛选 |
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| 引用本文： | 蒋世翠,张波,李艳双,李玉.黄柄钙皮菌无菌培养条件的优化筛选[J].菌物学报,2016,35(5):641-644. |
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| 作者姓名： | 蒋世翠 张波 李艳双 李玉 |
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| 作者单位： | 吉林农业大学食药用菌教育部工程研究中心 吉林 长春 130118 |
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| 基金项目： | 国家自然科学基金青年项目(3140010180)Supported by the National Natural Science Foundation of China for Yourth (3140010180). |
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| 摘 要： | 黄柄钙皮菌是黏菌中的一种重要模式生物，本文筛选了其无菌培养条件，比较了生活史不同阶段的发生时间，同时比较了生活史中孢子萌发、原质团生长及子实体形成3个阶段的培养条件。结果表明，黄柄钙皮菌适宜的培养条件为：在20%水琼脂上于28℃进行孢子萌发；在24–26℃下20%琼脂+40%燕麦培养基上进行原生质团的培养；在24–26℃下1 000Lx光照10–14h时形成子实体。
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| 关 键 词： | 黏菌 个体发育 无菌培养 |
Optimization of axenic culture conditions of Myxomycete Didymium iridis |
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| Authors: | JIANG Shi-Cui ZHANG Bo LI Yan-Shuang LI Yu |
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| Institution: | Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin 130118, China |
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| Abstract: | Axenic culture conditions of Didymium iridis (Myxogastrea) were screened and optimized, and occurrence time and culture conditions of the three growth stages (spore germination, plasmodium growth and fruiting body formation) of the slime mold were compared. The cultivation conditions of D. iridis were optimized as follows: spore germination on 20% agar at 28°C, plasmodium growth on medium containing 20% agar and 40% oat at 24–26°C, and fruiting body formation on medium containing 20% agar and 40% oat at 24–26°C under 1 000Lx illumination for 10–14h. |
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| Keywords: | slime molds individual development axenic culture |
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