采自武夷山高温木质素降解菌优良菌株筛选及其木质素降解效果测定

本文旨在筛选能够高效降解秸秆木质素的高温真菌。对来自福建武夷山的农田土壤进行富集,采用苯胺蓝、愈创木酚和α-萘酚3种筛选平板结合木质素磺酸钙降解试验筛选木质素高温降解菌,采用范氏洗涤剂法测定一株高效降解菌对秸秆木质素的降解效果;最后以经典形态学和多基因分子系统学相结合的方法对该菌株进行鉴定。结果表明：经钓饵法,分离获得8株高温菌;通过初筛和复筛,获得了1株较好的木质素高温降解菌A12638H;将其用于降解水稻秸秆和玉米秸秆,发现木质素降解率分别达到41.7%和48.3%;该菌株经鉴定为大孢戴氏霉Taifanglania major。菌株A12638H具有很好的应用价值,值得在秸秆资源的开发利用中开展更深入的研究。

Screening of superior lignin-degrading thermotolerant fungal strains from Wuyi Mountains and assessment of their degradation effects on straw lignin

Straw-lignin-degrading thermotolerant fungi were isolated from agricultural soils in Wuyi Mountains in Fujian Province. Three kinds of enrichment plate culture with addition of aniline blue, guaiacol and alpha-naphthol, combined with calcium lignosulfonate were used for lignin degradation experiment. The straw-lignin degradation ability was assessed by using Van-Soest detergent method. Identification of the fungi was based on morphology and phylogenetic analysis. Eight thermotolerant strains were obtained. Strain A12638H, identified as Taifanglania major, could effectively degrade straw lignin of rice and corn, with degradation rates of 41.7% and 48.3%, respectively. Strain A12638H has tremendous potential value for straw lignin degradation.