蝙蝠蛾幼虫肠道中的冬虫夏草菌体研究

西藏林芝地区冬虫夏草天然产区野生虫草蝙蝠蛾幼虫的肠道液pH范围为9.69-10.99,体外在此pH下培养冬虫夏草菌长势较差。同时,在该pH的体外模拟中肠液中,虫菌体也会在极短的时间内消亡,而对自然侵染的蝙蝠蛾幼虫的血淋巴、消化道及其“反吐物”（肠液）进行切片和显微镜检却发现,蝙蝠蛾幼虫消化道、呕吐物中均存在与血淋巴中相同的冬虫夏草菌菌体形态,生存状态良好,正常增殖,表明蝙蝠蛾幼虫肠道的生理状态可以维系并支撑冬虫夏草菌菌体某种形态的存在与生长,并证实冬虫夏草菌由口器及消化道入侵蝙蝠蛾幼虫的可能性;肠道虫菌体与血淋巴虫菌体不一定同时存在,表明冬虫夏草菌对蝙蝠蛾幼虫的侵染可能同时存在表皮侵染和肠道侵染两种途径。本文为冬虫夏草菌对蝙蝠蛾幼虫的侵染途径研究提供更加多样化的信息支持。

Ophiocordyceps sinensis in the intestines of Hepialus larvae

The intestinal juice pH of wild Hepialus larvae in cordyceps natural production areas in Nyingchi of Tibet was between 9.69 and 10.99. Under this pH, Ophiocordyceps sinensis grew in undesirable way in vitro. In mimic intestinal juice under this pH, the thalli died out within a very short time. However, microscopic examination of paraffin section of hemolymph, digestive tract and “vomit” (intestinal juice) of naturally infected Hepialus larvae showed that the same cordyceps fungus was found to be existed in digestive tract and “vomit” as in hemolymph, and the fungus was in good condition and propagated normally, indicating that the physiological state of Hepialus larval gut could maintain and support the fungus existing and growing in a certain form, and confirming the possibility that the Hepialus larvae could be infected by O. sinensis via mouth and digestive tract. The intestinal thalli and hemolymph thalli might not exist at the same time. It is suggested that the larvae may be infected by the way of skin infection and intestinal infection.