培养基及培养条件对冬虫夏草菌固体发酵产分生孢子的影响

为获得冬虫夏草菌固体发酵产分生孢子的最优工艺,以野生分离的冬虫夏草菌为材料,对其固体发酵产分生孢子的培养基及培养条件进行了研究。试验结果表明：泥炭土为最佳基础培养基,该培养基中冬虫夏草菌气生菌丝生长一般,但产分生孢子最多,可达4.2×10³个/g;泥炭土培养基中添加0.1‰ IAA（吲哚乙酸）、0.1‰ IBA（吲哚丁酸）和0.1‰ NAA（萘乙酸）能促进冬虫夏草菌气生菌丝的生长和分生孢子的产生,其分生孢子达8.1×10³个/g;该基础培养基中,冬虫夏草菌于18℃培养30d后,在10℃、相对湿度45%、蓝光照射进行诱导,分生孢子可达1.0×10⁴个/g。本研究建立了一种大量获取冬虫夏草菌分生孢子的方法,为冬虫夏草繁育奠定了基础。

Effects of medium and environmental conditions on the sporulation of Ophiocordyceps sinensis in solid fermentation

The medium and environmental conditions for the sporulation of Ophiocordyceps sinensis in solid fermentation were optimized. Among the five basical media tested, peat soil medium is the best for the sporulation. In this medium, the mycelia grow ordinarily, but produce huge amount of conidia, reaching 4.2×10³/g. Indole acetic acid (IAA), indole butyric acid (IBA) and naphthaleneacetic acid (NAA) can stimulate the growth of mycelium. Adding 0.1‰ IAA, 0.1‰ IBA and 0.1‰ NAA to the peat soil medium, the mycelia can yield 8.1×10³/g of conidia. After culturing at 18°C in 30d and stimulating under low temperature 10°C, 45% humidity and blue light illumination, the mycelia can produce 1.0×10⁴/g of conidia. The result demonstrates that preparation of huge amount of Ophiocordyceps sinensis conidia is feasible, and lays the foundation of Ophiocordyceps sinensis breeding.