红托竹荪多糖诱导肿瘤细胞凋亡的作用初探

探讨红托竹荪多糖对小鼠腹水瘤S180细胞凋亡的作用。分别使用不同浓度的红托竹荪多糖处理S180细胞24h,Western blot法检测Bcl-xl、Bax、caspase-9和Caspase-3等蛋白表达,使用流式细胞仪检测细胞凋亡。Western blot 法检测结果显示Bcl-xl表达量随竹荪多糖剂量增加而降低,Bax表达量则相反,每组Bax/Bcl-xl的比值增加,Caspase-9、Caspase-3表达量增加;流式细胞仪检测结果显示：0、25、50和100mg/L组细胞凋亡率分别为5.12%±0.11%、9.61%±0.61%、16.39%±0.19%和17.05%±0.13%,与对照组相比细胞凋亡率增高,差异具有统计学意义（P<0.05）,推断红托竹荪多糖具有诱导S180细胞凋亡的功能。

Induction of Dictyophora rubrovolvata polysaccharides on tumor cell apoptosis

Different concentrations of Dictyophora rubrovolvata polysaccharides were used to treat ascitic tumor S180 cells for 24h to investigate the effect of the polysaccharides on tumor cell apoptosis. Western blotting analysis was performed to detect the expressions of Bcl-xl, Bax, caspase-9 and caspase-3 proteins, and the flow cytometry assay was conducted to detect cell apoptosis. Western blot test results showed that the Bcl-xl expression decreased with the increase of concentration of Dictyophora rubrovolvata polysaccharides. On the contrary, Bax expression quantity, the ratio of Bax/Bcl-xl, Caspase-9 and Caspase-3 expression quantity of each treated group increased. Flow cytometry test results showed that four dose, 0, 25, 50 and 100mg/L of polysaccharides resulted in the apoptosis rate of 5.12%±0.11%, 9.61%±0.61%, 6.39%±0.19% and 17.05%±0.13%, respectively. Compared with control group, apoptosis rate was increased in a dose-dependent manner. The differences were statistically significant (P<0.05). Thus, Dictyophora rubrovolvata polysaccharides could induce S180 cell apoptosis.