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| 培养条件对头孢霉脂肪酸链长和ω-3脱饱和的影响 | |
| 引用本文： | 戴传超,余伯阳,袁生,李霞,陆玲.培养条件对头孢霉脂肪酸链长和ω-3脱饱和的影响[J].菌物学报,2001,20(2):201-206. |
| 作者姓名： | 戴传超 余伯阳 袁生 李霞 陆玲 |
| 作者单位： | 1. 南京师范大学生命科学学院 中国药科大学 2. 中国药科大学 3. 南京师范大学生命科学学院 4. 南京师范大学生命科学学院 江苏省农业科学院遗传生理研究所 |
| 基金项目： | 江苏省青年基金!(BQ96025)，南京师范大学理科青年基金资助 |
| 摘 要： | 为了获得高产量的长链ω-3多不饱和脂肪酸，用十八碳脂肪酸和十六碳脂肪酸的比值考察碳链延长，用α-亚麻酸和亚油酸的比值考察ω-3脱饱和；探讨了八种因子对脂肪酸链长和ω-3脱饱和的影响。有利于碳链延长的条件为：麦芽糖10g/L、(NH4)2SO4 3g/L、起始pH为4.0、500mL三角瓶装50mL培养基、接种20%（V/V）、20℃培养6d。有利于ω-3脂肪酸生成的条件为：蔗糖30g/L、NH4Cl 3g/L、培养基起始pH为4.0、500mL三角瓶装50mL培养基、接种20%（V/V）、10℃培养10d。 |
| 关 键 词： | 头孢霉(Cephalosporium sp.) 脂肪酸碳链延长 脂肪酸ω-3脱饱和 |
| 文章编号： | 1007-3515（2001）02-0201-0206 |
| 修稿时间： | 2000年9月20日 |
THE EFFECTS OF CULTURE CONDITIONS ON THE CHAIN ELONGATION AND |
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| Authors: | DAI Chuan-Chao YUAN Sheng LI Xia LU Ling YU Bo-Yang |
| Institution: | DAI Chuan-Chao1,2 YUAN Sheng1 LI Xia1 LU Ling1 YU Bo-Yang2 |
| Abstract: | The effects of eight factors on fatty acid chain elongation andω-3 desaturation were studied in the culture of Cephalosporium sp. for increasing yields of long chainω-3 polyunsaturated fatty acids. The ratio of ΣC18 and ΣC16 fatty acids was used to estimate the elongation of fatty acids; the ratio of α-linolenic acid and linoleic acid was used to the ω-3 desaturation. The results shows that the elongation of fatty acids could be enhanced by some culture conditions including maltose 10g/L、(NH4)2SO4 3g/L、medium's initial pH 4.0、50ml medium in 500ml flask、seeding 20% (v/v%) and culturing it for 6 days in 20℃. Whereas the ω-3 desaturation of fatty acids was favorable in following culture conditions: sucrose 30g/L、NH4Cl 3g/L、medium's initial pH 4.0、50ml medium in 500ml flask、seeding 20% (v/v%) and culturing it for 10 d in 10℃. |
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