Effect of the dnaN mutation on the growth of small DNA phages |
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Authors: | A Taketo |
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Affiliation: | (1) Department of Biochemistry I, Fukui Medical School, Matsuoka, 910-11 Fukui, Japan;(2) Department of Biochemistry II, School of Medicine, Kanazawa University, 920 Kanazawa, Ishikawa, Japan |
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Abstract: | Summary The effect of the dnaN mutation on the growth of single-stranded DNA phages was studied by burst experiments. In HC138 dnaN cells exposed to 42.5° C at 5 min before infection, growth of spherical (microvirid or isometric) phages such as 3, Kh-1 and X174 was partially reduced at the nonpermissive temperature. When infection was performed at 30 min after temperature shift-up, viral replication was completely inhibited at 42.5° C in the dnaN strain but not in a dna+ revertant. At 41° C, multiplication of filamentous (inovirid) phages M13 and fd was restricted specifically in HC138 F+dnaN bacteria. When dnaN cells lysogenic for i21 were grown at 42.5° C for 60 min and then shifted down to 33° C, a burst of i21 occurred with concomitant cellular lysis, manifesting induction of the prophage development. |
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