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Growth characteristics and ion contents of non-selected and salt-selected callus lines of highbush blueberry (Vacdnium corymbosum) cultivars Blue Crop and Denise Blue
Authors:Morley S. Muralitharan  Reinhard F. M. Van Steveninck  Stephen E Chandler
Affiliation:(1) School of Agriculture, La Trobe University, 3083 Bundoora, Australia;(2) Calgene Pacific, 16 Gipps Street, 3066 Collingwood, Australia
Abstract:Non-selected and sodium chloride selected callus lines of Vacdnium corymbosum L.cv Blue Crop and cv. Denise Blue were grown on media supplemented with 0–100 mM NaCl. For both cultivars, fresh weight and dry weight yields were greater in selected lines on all levels of NaCl. Selected lines of Blue Crop displayed better growth than selected lines of Denise Blue at most concentrations of NaCl. Internal Na+ and Cl concentrations in selected and non-selected lines of both cultivars increased as external concentration was raised. However, selected lines of Blue Crop and Denise Blue accumulated more Na+ and Cl than non-selected lines. Selected lines of both cultivars maintained higher levels of K+ than non-selected lines on all external NaCl levels. Selected lines of Blue Crop had higher levels of Na+ and Cl than that of Denise Blue. The results suggest Na+ and Cl accumulation could be a mechanism allowing better growth in selected lines at moderate salinity levels (50–75 mM NaCl).
Keywords:Salinity  invitro  ions  blueberry  Vaccinium corymbosum
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