Alterations in intracellular calcium homeostasis and platelet aggregation induced by ethanol |
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Authors: | Rosado Juan A Núñez Ana M Pariente José A Salido Ginés M |
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Affiliation: | Department of Physiology, University of Extremadura, 10071 Cáceres, Spain. jarosado@unex.es |
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Abstract: | The in vitro effects of ethanol on intracellular Ca(2+) homeostasis and tyrosine phosphorylation have been investigated in human platelets in order to clarify the cellular mechanisms underlying its described anti-aggregant effects. Ethanol (1-50 mM) reduced, in a dose-dependent manner, the rate and amplitude of aggregation and attenuated the phosphotyrosine content both induced by 0.1U/ml of the physiological ligand, thrombin. Thrombin-induced Ca(2+) entry to the cytosol was significantly reduced, and capacitative Ca(2+) entry (CCE) significantly altered, by 50 mM ethanol, so that ethanol reduces CCE mediated by depletion of the 2,5-di-(tert-butyl)-1,4-hydroquinone (TBHQ)-sensitive store but enhances CCE induced by the TBHQ-insensitive pool. In conclusion, we provide considerable evidence that ethanol reduces thrombin-induced aggregation, which is likely a result of a significant inhibition of Ca(2+) entry, as well as a reduction in the activity of protein tyrosine kinases. |
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Keywords: | Ca2+ entry PMCA Platelets Thrombin Ethanol Aggregation |
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