Quantification of annexin I in subcellular fractions of human neutrophils reveals an exclusive cytosolic localisation |
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| Authors: | Movitz C Dahlgren C |
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| Institution: | 1. Research Institute of Advanced Energy Technology, Kyungpook National University, Daegu 41566, Republic of Korea;2. Department of Chemical Engineering, Kyungpook National University, Daegu 41566, Republic of Korea;3. Institute for Advanced Engineering, Yongin 41718, Republic of Korea;4. Research Institute of Industrial Science and Technology, Pohang 37673, Republic of Korea;5. Construction Engineering Service Co., LTD., Gunpo 15850, Republic of Korea |
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| Abstract: | Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory gelatinase granules of human neutrophils, suggesting that the protein might be released through a granule mobilisation and fusion process resembling classical secretion. In this study we have determined the intracellular localisation of annexin I in human neutrophils using subcellular fractionation, protein separation by SDS-PAGE and immunoblotting, and show that virtually all annexin I is localised in the cell cytosol. |
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| Keywords: | annexin I human neutrophils subcellular localisation secretion |
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