Highly effective detection of inflamed cells using a modified bradykinin ligand labeled with FITC fluorescence |
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| Institution: | 1. Department of Biotechnology and Bioinformatics, Korea University, Sejong-Ro 2511, Sejong 339-700, Republic of Korea;2. Department of Chemical Engineering, Chungnam National University, Daejeon 305-764, Republic of Korea;1. Interdisciplinary Graduate School of Engineering Sciences, Kyushu University, 6-1 Kasuga-koen, Kasuga-shi, Fukuoka 816-8580, Japan;2. Sumika Agrotech Co., Ltd., 5-1 Sobiraki-cho, Nihama-shi, Ehime 792-0001, Japan;1. Department of Food Hygiene and Technology, Faculty of Veterinary Science, University of Leon, 24071, León, Spain;2. Department of Nutrition, Faculty of Nutrition, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil;1. Academic Unit of Respiratory Medicine, University of Sheffield, The Medical School (Floor L), Beech Hill Road, Sheffield S10 2RX, UK;2. Department of Histopathology, Royal Hallamshire Hospital, Glossop Road, Sheffield S10 2JF, UK;1. Laboratory of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga, Shizuoka 422-8526, Japan;2. Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Amphur Maung, Khon Kaen, Thailand;3. Shizuoka Kensan Shoyu K. K., 80-1 Takashinden, Yaizu, Shizuoka 421-0204, Japan;1. Institute of Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany;2. Research Core Unit Metabolomics, Hannover Medical School, Carl-Neuberg-Str. 1, D-30625 Hannover, Germany |
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| Abstract: | Detection of inflammation in live cells is important because long-lasting inflammation is considered to be a primary cause of several diseases. However, few reports have been published on imaging analysis of inflammation in live cells. In this study, we developed an effective imaging system for detection of inflamed cells using a bradykinin ligand (BK) or a modified BK (mBK), which has specific affinity with the cellular B1R receptor. Synthetic BK or mBK labeled with FITC at the N-terminus was employed for discriminating between inflamed and normal cells; this method was found to be effective for detection of inflammation in live cells. In addition, using the mBK-based cell imaging system, we successfully performed flow-based analysis of live cell inflammation on a micro-chip channel, composed of a Starna flow cell and PDMS (Polydimethylsiloxane) walls. The BK-based cell imaging methods designed here would be a useful platform for development of a high-throughput live cell analysis system for investigating the factors underlying inflammation or for screening of anti-inflammation candidate drugs. |
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| Keywords: | Inflammation Bradykinin Live cell imaging Flow-based cell analysis |
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