In vivo synthesis of nano-selenium by Tetrahymena thermophila SB210 |
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| Affiliation: | 1. CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, China;2. Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China;3. Engineering and Materials Science Experiment Center, University of Science & Technology of China, Hefei 230026, China;1. Dairy Microbiology Division, National Dairy Research Institute, Karnal, Haryana 132001, India;2. Department of Dairy Microbiology, College of Dairy Technology, Chhattisgarh Kamdhenu Vishwavidyalya, Raipur, Chhattisgarh 492006, India;3. Department of Food Science, University of Missouri, USA;4. Directorate of Knowledge Management in Agriculture, Indian Council of Agricultural Research, New Delhi, India;1. Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;2. Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;1. Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China;2. Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian 116024, China;1. Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic;2. Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic;3. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic |
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| Abstract: | Nano-selenium has a great potential to be used in chemical, biological, medical and environmental fields. Biological methods for nano-selenium synthesis have attracted wide interests, because they can be operated at ambient temperature and pressure without complicated equipments. In this work, a protozoa, Tetrahymena thermophila (T. thermophila) SB210, was used to in vivo synthesize nano-selenium. The biosynthesized nano-selenium was characterized using transmission electron microscopy, energy dispersive X-ray spectroscopy and Raman spectroscopy. The synthesized amorphous spherical selenium nanoparticles had diameters of 50–500 nm with the coexistence of irregular nano-selenium. The expressions of glutathione (GSH) synthesis related gene glutathione synthase, cysteine-rich protein metallothionein related gene metallothionein-1 and [2Fe-2S] cluster-binding protein related gene were up-regulated in the nano-selenium producing group. Also, the subsequent GSH detection and in vitro synthesis experimental results suggest the three proteins were likely to be involved in the nano-selenium synthesis process. |
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| Keywords: | Nano-selenium Animal Biosynthesis Glutathione (GSH) Proteins |
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