Discovery and characterization of new O-methyltransferase from the genome of the lignin-degrading fungus Phanerochaete chrysosporium for enhanced lignin degradation |
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| Institution: | 1. Department of Forest Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-921, Republic of Korea;2. Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 232-916, Gangwon-do, Republic of Korea;1. Cátedra de Química Orgánica, Facultad de Ciencias Exactas, Físicas y Naturales (FCEFyN), Universidad Nacional de Córdoba (UNC), Av. Vélez Sarsfield 1611, Córdoba, X5016GCA, Argentina;2. Instituto Multidisciplinario de Biología Vegetal (IMBiV-CONICET), UNC. Av. Vélez Sarsfield 1611, Córdoba, X5016GCA, Argentina;3. Instituto de Ciencia y Tecnología de los Alimentos (ICTA), FCEFyN-UNC. Av. Vélez Sarsfield 1611, Córdoba, X5016GCA, Argentina;4. Facultad de Ciencias Agropecuarias (FCA), Universidad Nacional de Córdoba, Ing. Agr. Félix Aldo Marrone 746, Córdoba, X5016GCA, Argentina;5. Laboratorio de Biotecnología Fúngica y de los Alimentos, Asociación para el Desarrollo de Villa Elisa y Zona, Héctor de Elia 1247, Villa Elisa (Entre Ríos), E3265, Argentina;1. Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, Belgrade, Serbia;2. Molecular Enzymology Group, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands;1. Department of Biotechnology and Life Sciences, Università degli Studi dell’Insubria, via J.H. Dunant 3, 21100, Varese, Italy;2. The Protein Factory, Politecnico di Milano and Università degli Studi dell’Insubria, via Mancinelli 7, 20131, Milano, Italy;3. Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, p.zza Leonardo da Vinci 32, 20133, Milano, Italy;1. Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring, 17, 35392 Giessen, Germany;2. Department of Organic Chemistry, Igor Sikorsky Kyiv Polytechnic Institute, Peremogy Ave., 37, 03056 Kyiv, Ukraine;3. Fraunhofer Institute for Molecular Biology and Applied Ecology, Ohlebergsweg 12, 35392 Giessen, Germany |
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| Abstract: | Using bioinformatic homology search tools, this study utilized sequence phylogeny, gene organization and conserved motifs to identify members of the family of O-methyltransferases from lignin-degrading fungus Phanerochaete chrysosporium. The heterologous expression and characterization of O-methyltransferases from P. chrysosporium were studied. The expressed protein utilized S-(5′-adenosyl)-l-methionine p-toluenesulfonate salt (SAM) and methylated various free-hydroxyl phenolic compounds at both meta and para site. In the same motif, O-methyltransferases were also identified in other white-rot fungi including Bjerkandera adusta, Ceriporiopsis (Gelatoporia) subvermispora B, and Trametes versicolor. As free-hydroxyl phenolic compounds have been known as inhibitors for lignin peroxidase, the presence of O-methyltransferases in white-rot fungi suggested their biological functions in accelerating lignin degradation in white-rot basidiomycetes by converting those inhibitory groups into non-toxic methylated phenolic ones. |
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| Keywords: | White-rot fungi Lignin peroxidase Free-phenolic compounds |
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