Nutrient control for stationary phase cellulase production in Trichoderma reesei Rut C-30 |
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| Affiliation: | 1. State Key Laboratory of Microbial Technology and National Glycoengineering Research Center, Shandong University, Jinan 250100, China;2. School of Life Science, Qufu Normal University, Qufu 273165, China;1. Faculty of Bioscience and Medical Engineering, Department of Biotechnology and Medical Engineering, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Malaysia;2. School of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310, Johor Bahru, Malaysia;3. Innovation Centre in Agritechnology for Advanced Bioprocessing, UTM Pagoh, Hub Pendidikan Tinggi Pagoh, 84600, Pagoh, Johor. Malaysia;4. Department of Chemistry/Biochemistry, Federal University Ndufu-Alike Ikwo, PMB, 1010, Ebonyi State, Nigeria;5. Natural Products and Drug Discovery Center, Malaysian Institute of Pharmaceuticals and Nutraceuticals, National Institute of Biotechnology Malaysia, Ministry of Science, Technology and Innovation, Block 5-A, Halaman Bukit Gambir, 11700, Pulau Pinang, Malaysia;6. Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Malaysia;7. Enzyme Technology and Green Synthesis Group, Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Malaysia;1. Academy of Scientific and Innovative Research (AcSIR), CSIR-NIIST Campus, Thiruvananthapuram, India;2. Centre for Biofuels, Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Industrial Estate P.O., Thiruvananthapuram 695019, India;3. Centre for Innovative and Applied Bioprocessing, Mohali 160 071, Punjab, India;4. Indian Institute of Millets Research, Hyderabad, India;1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;2. State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;3. Department of Food, Agricultural and Biological Engineering, The Ohio State University/Ohio Agricultural Research and Development Center, Wooster, OH 44691-4096, USA;1. Department of Biochemistry and Chemical Technology, IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55, 14800-060 Araraquara, São Paulo State, Brazil;2. Department of Biology, IBILCE/UNESP–Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, São Paulo State, Brazil;3. Department of Chemistry and Environmental Sciences, IBILCE/UNESP – Univ Estadual Paulista, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, São Paulo State, Brazil;4. Department of Organic Chemistry, IQ/UNESP–Univ Estadual Paulista, Rua Prof. Francisco Degni, 55, 14800-060 Araraquara, São Paulo State, Brazil;5. Department of Biochemistry and Microbiology, IB/UNESP–Univ Estadual Paulista, Avenida 24A, 1515, 13506-900, Rio Claro, São Paulo State, Brazil |
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| Abstract: | This work describes the use of nutrient limitations with Trichoderma reesei Rut C-30 to obtain a prolonged stationary phase cellulase production. This period of non-growth may allow for dependable cellulase production, extended fermentation periods, and the possibility to use pellet morphology for easy product separation. Phosphorus limitation was successful in halting growth and had a corresponding specific cellulase production of 5 ± 2 FPU/g-h. Combined with the addition of Triton X-100 for fungal pellet formation and low shear conditions, a stationary phase cellulase production period in excess of 300 h was achieved, with a constant enzyme production rate of 7 ± 1 FPU/g-h. While nitrogen limitation was also effective as a growth limiter, it, however, also prevented cellulase production. |
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| Keywords: | Cellulase Phosphorus Nitrogen Pellet |
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