Electrospun polyvinyl alcohol/bovine serum albumin biocomposite membranes for horseradish peroxidase immobilization |
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| Institution: | 1. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran;2. Nanobiotechnology Engineering Laboratory, Faculty of Engineering and New Technologies, Shahid Beheshti University, GC, Tehran, Iran;3. Protein Engineering Laboratory, Protein Research Center (PRC), Shahid Beheshti University, GC, Tehran, Iran;4. Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran;1. Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China;2. Department of Hematology, The First Affiliated Hospital of Xi''an Jiaotong University, Xi''an 710061, China;1. Nanotechnology Institute, Amirkabir University of Technology, Tehran, Iran;2. Textile Engineering Department, AmirKabir University of Technology, Tehran, Iran;3. Biomaterials Group, Biomedical Engineering Department, Amirkabir University of Technology, Tehran, Iran;4. Biosensors & Bioelectronics Centre, Dept. of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden;1. Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, M?egyetem rkp. 3, H-1111 Budapest, Hungary;2. Fermentia Ltd., Berlini út. 47-49, H-1049, Budapest, Hungary;3. SynBiocat Ltd., Löv?ház u 19/1, H-1024 Budapest, Hungary;4. Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Budafoki út 8, H-1111 Budapest, Hungary;5. Agricultural Institute, Centre of Agricultural Research, Hungarian Academy of Sciences, Brunszvik u. 2, H-2462 Martonvásár, Hungary;6. Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary;1. Univ Lyon, CNRS, Université Claude Bernard Lyon 1, ENS de Lyon, Institute of Analytical Sciences, UMR 5280, 5 Rue la Doua, F-69100 Villeurbanne, France;2. University of Monastir, Laboratoire des Interfaces et des Matériaux Avancés, Faculté des Sciences de Monastir, Avenue de l’Environnement, 5019 Monastir, Tunisia;3. Univ Lyon, Université Jean Monnet Saint-Etienne, CNRS, Laboratory Hubert Curien, UMR 5516, 18 rue Professeur Benoit Lauras, F-42000 Saint-Etienne, France;4. Univ Lyon, Université Claude Bernard Lyon 1, CNRS, Institut des Nanotechnologies de Lyon, UMR5270, Bâtiment Léon Brillouin, 6 rue Ampère, F-69622 Villeurbanne cédex, France;1. Department of Mechanical Engineering, North Dakota State University, Fargo, ND 58108, United States;2. Program of Materials and Nanotechnology, North Dakota State University, Fargo, ND 58108, United States;3. Department of Statistics, North Dakota State University, Fargo, ND 58108, United States |
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| Abstract: | Electrospinning, a simple and versatile method to fabricate nanofibrous supports, has attracted attention in the field of enzyme immobilization. Biocomposite nanofibers were fabricated from mixed PVA/BSA solution and the effects of glutaraldehyde treatment, initial BSA concentration and PVA concentration on protein loading were investigated. Glutaraldehyde cross-linking significantly decreased protein release from nanofibers and BSA loading reached as high as 27.3% (w/w). In comparison with the HRP immobilized into the nascent nanofibrous membrane, a significant increase was observed in the activity retention of the enzyme immobilized into the PVA/BSA biocomposite nanofibers. The immobilized HRP was able to tolerate much higher concentrations of hydrogen peroxide than the free enzyme and thus the immobilized enzyme did not demonstrate substrate inhibition. The immobilized HRP retained ?50% of the free enzyme activity at 6.4 mM hydrogen peroxide and no significant variation was observed in the KM value of the enzyme for hydrogen peroxide after immobilization. In addition, reusability tests showed that the residual activity of the immobilized HRP were 73% after 11 reuse cycles. Together, these results demonstrate efficient immobilization of HRP into electrospun PVA/BSA biocomposite nanofibers and provide a promising immobilization strategy for biotechnological applications. |
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| Keywords: | Biocomposite nanofibrous membranes Horseradish peroxidase Immobilization Electrospinning Cross-linking Bovine serum albumin |
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