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Ethanol/O2 biofuel cell using a biocathode consisting of laccase/ HOOC-MWCNTs/polydiallyldimethylammonium chloride
Institution:1. Departamento de Ciencias Ambientales, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Lerma, Av. de las Garzas No. 10, Col. El Panteón, Lerma de Villada, Municipio de Lerma, Estado de México, C.P. 52005, Mexico;2. Division de Ciencias Naturales e Ingeneria, Universidad Autónoma Metropolitana-Cuajimalpa, Artificios 40, Col. Hidalgo, Del. Álvaro Obregón C.P. 01120, México, D.F., Mexico;3. Nuclear Physics Institute, 25068 Řež, Czech Republic;4. Departamento de Fisica, Universidad Autónoma Metropolitana-Iztapalapa, PO Box 55-534, 09340 México, D.F., Mexico;5. Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva 84105, Israel;1. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore;2. Department of Biomedical Engineering, National University of Singapore, Singapore;3. Department of Surgery, National University of Singapore, Singapore;1. University of Amsterdam, van’t Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands;2. Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, B-1050 Brussels, Belgium;1. Dow Benelux, Analytical Sciences, P.O. Box 48, 4530 AA Terneuzen, The Netherlands;2. University of Amsterdam, Van ‘t Hoff Institute for Molecular Sciences, Science Park 904, 1098 XH Amsterdam, The Netherlands;3. Dutch Polymer Institute, P.O. Box 902, 5600 AX Eindhoven, The Netherlands;4. The Dow Chemical Company, Analytical Sciences, 1897 Building, Midland, MI 48667, USA;1. Endoscopy Unit, Department of Digestive Diseases, Hospital Universitari de Bellvitge-IDIBELL, Barcelona, Catalonia, Spain;2. Endoscopy Unit, Department of Digestive Diseases, Hospital Clinic San Carlos, Madrid, Spain;3. Endoscopy Unit, Department of Digestive Diseases, Hospital Santa Creu i Sant Pau, Barcelona, Catalonia, Spain;4. Endoscopy Unit, Department of Digestive Diseases, Hospital Universitario Araba sede Txagorritxu, Alava, Spain;5. Endoscopy Unit, Department of Digestive Diseases, Hospital Virgen de la Salud, Toledo, Spain;6. Endoscopy Unit, Department of Digestive Diseases, Hospital Clinic, Barcelona, Catalonia, Spain;7. Endoscopy Unit, Department of Digestive Diseases, Hospital Mútua Terrassa, CIBEREHD, Terrassa, Catalonia, Spain
Abstract:In the present report we focused on the substitution of metallic catalysts by biocatalysts to develop a high efficient biofuel cell. A bioanode and a biocathode were designed using ADH and laccase, respectively. Carboxylated multiwall carbon nanotubes (HOOC-MWCNTs) and polydiallyldimethylammonium chloride (PDDA) were used for immobilizing the enzymes on either polymethylene green (PMG) modified glassy carbon or graphite electrodes. In this way, an ethanol–oxygen biofuel cell was designed in which PDDA/ADH/PDDA/HOOC-MWCNTs/PMG/GC and PDDA/Lac/PDDA/HOOC-MWCNTs/PMG/Gr operated as bioanode and biocathode, respectively. In the optimized condition of O2 saturated PBS (0.1 M, pH 7.5) containing 1 mM ethanol and 1 mM NAD+ the open-circuit voltage reached to a plateau at 504 mV based of which the power density of 3.98 mW cm−2 was obtained.
Keywords:Biofuel cell  Polydiallyldimethylammonium chloride  Carboxylated multiwall carbon nanotube  Laccase  Alcoholdehydrogenase  Polymethylene green
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