Effects of additives on efficiency and specificity of ligase detection reaction |
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| Authors: | Zhen-Xian Xiao Hui-Min Cao Xiao-Hui Luan Jian-Long Zhao Dong-Zhi Wei Jun-Hua Xiao |
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| Affiliation: | (1) Institute of Biological Science and Technology, Donghua University, 2999 North Renmin Road, 201620 Shanghai, Songjiang District, P.R. China;(2) State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 200237 Shangh, P.R. China;(3) Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Science, 20050 Shanghai, P.R. China |
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| Abstract: | Ligase detection reaction (LDR) is adaptable to a wide variety of applications ranging from scientific research to clinical diagnosis, especially in the field of nucleotide polymorphism discrimination and analysis. Efficiency and specificity of LDR are the most two important characteristics that influence its application. To improve the specificity or efficiency of ligase, optimization of the design of LDR probes and the reaction of LDR were investigated previously by most researchers. But the effects of additives on LDR have not been reported. In this study, the effects of additives (DMSO, Tween-20, glycerol, formamide, and PEG-6000) on LDR efficiency and specificity were investigated. The results showed that all of these compounds, except for Tween-20, could improve the specificity of LDR. PEG-6000 was proved to be the best additive among the five tested with an optimal concentration of 5% at which the highest yield was obtained with a relatively improved specificity. |
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| Keywords: | LDR additives efficiency specificity |
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