Functional avidity of tumor antigen-specific CTL recognition directly correlates with the stability of MHC/peptide multimer binding to TCR. |
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Authors: | Valérie Dutoit Verena Rubio-Godoy Marie-Agnès Doucey Pascal Batard Danielle Liénard Donata Rimoldi Daniel Speiser Philippe Guillaume Jean-Charles Cerottini Pedro Romero Danila Valmori |
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Affiliation: | Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, H?pital Orthopédique, Niveau 5, Avenue Pierre-Decker 4, CH 1005 Lausanne, Switzerland. |
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Abstract: | Avidity of Ag recognition by tumor-specific T cells is one of the main parameters that determines the potency of a tumor rejection Ag. In this study we show that the relative efficiency of staining of tumor Ag-specific T lymphocytes with the corresponding fluorescent MHC class I/peptide multimeric complexes can considerably vary with staining conditions and does not necessarily correlate with avidity of Ag recognition. Instead, we found a clear correlation between avidity of Ag recognition and the stability of MHC class I/peptide multimeric complexes interaction with TCR as measured in dissociation kinetic experiments. These findings are relevant for both identification and isolation of tumor-reactive CTL. |
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