| miR-143 通过抑制PTN 促进人骨髓间充质干细胞成脂分化 |
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| 引用本文： | 伊璨,谢伟东 吕青,张雅鸥. miR-143 通过抑制PTN 促进人骨髓间充质干细胞成脂分化[J]. 现代生物医学进展, 2011, 11(18): 3401-3404 |
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| 作者姓名： | 伊璨 谢伟东 吕青 张雅鸥 |
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| 作者单位： | 1. 清华大学生命科学学院 北京100084;清华大学深圳研究生院生命与健康学部 广东深圳518055
2. 清华大学深圳研究生院生命与健康学部 广东深圳518055 |
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| 摘 要： | 目的:研究miR-143调控人骨髓间充质干细胞(hMSCs)成脂分化的新机理。方法:将NC、miR-143、siPTN、miR-143i转入hMSCs中,诱导成脂分化,检测miR-143对成脂分化的影响。经miRNA靶点分析软件Findtar预测出miR-143在人多效生长因子(hPTN)的3’-UTR端有靶点。RT-PCR、western blot研究miR-143与hPTN的关系。构建hPTN 3’-UTR靶位点荧光检测质粒prltk-PTN及其突变质粒prltk-m,验证miR-143是否在人PTN 3’-UTR上有靶点。结果:miR-143促进hMSCs成脂分化,抑制hPTN的mRNA和蛋白表达水平。荧光报告实验证实miR-143在人PTN的3’-UTR上有靶点。结论:miR-143通过与hPTN3’-UTR上的靶点相结合而抑制hPTN的表达,从而促进了hMSCs成脂分化进程。
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| 关 键 词： | miR-143 hPTN 成脂分化 hMSCs |
miR-143 Accelerates hMSCs Adipogenesis by Suppressingthe Expresssion of PTN |
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| YI Can,XIE Wei-dong,LV Qing,ZHANG Ya-ou. miR-143 Accelerates hMSCs Adipogenesis by Suppressingthe Expresssion of PTN[J]. Progress in Modern Biomedicine, 2011, 11(18): 3401-3404 |
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| Authors: | YI Can XIE Wei-dong LV Qing ZHANG Ya-ou |
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| Affiliation: | YI Can1,2,XIE Wei-dong2,LV Qing1,ZHANG Ya-ou2(1 School of Life Science,Tsinghua University,Beijing 100084,China,2 Division of Life Science,Graduate School at Shenzhen,Shenzhen,518055,China) |
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| Abstract: | Objective: To investigate the mechanism of miR-143 in the regulation of hMSCs adipogenesis.Methods: hMSCs were transfected with NC/miR-143/siPTN/miR-143i and induced into adipogenic differentiation to detect the effect of miR-143 on adipogene-sis.The miR-143 target in 3'-UTR of hPTN was predicted by Findtar-software of miRNA.The relationship between miR-143 and hPTN was researched by the methods of RT-PCR and western blot.The target of miR-143 predicted in hPTN 3'-UTR was verified by luciferase expression o... |
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| Keywords: | miR-143 hPTN Adipogenesis hMSCs |
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